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A Look at the Different Parts of a Spectrometer

Spectrometers are complex instruments that use the interaction of light and matter to perform qualitative and quantitative analysis of samples. But what are the main components inside these devices that allow them to function? In this article, we will explore the different working parts of spectrometers and how they enable these machines to do their job.

1. The Light Source

The light source is the beginning of the whole process. Just like our eyes need light to see, spectrometers require light to ‘see’ and analyze samples. Early spectrometers used broad-spectrum white light sources like tungsten lamps. Modern instruments employ monochromators to generate light of a specific wavelength. Common light sources in spectrometers include:

Tungsten Lamps

Tungsten filament lamps provide a continuous spectrum of light ranging from ultraviolet, and visible to infrared wavelengths when heated to high temperatures. Think of these as very bright incandescent light bulbs inside the machine.

Light Emitting Diodes

LEDs generate narrow bandwidth light centered around specific wavelengths. LEDs are compact, durable, and efficient compared to other sources. However, their narrow emission range requires additional components if a broader spectrum is needed.


Lasers are intense beams of light at a single wavelength. Helium-neon and argon lasers are commonly used in spectrometers when high energy over a very narrow range is required. The focused output of lasers can also be steered with greater precision.


These optical devices isolate a desired wavelength band from a broader light source like a tungsten lamp. Using prisms or diffraction gratings, monochromators filter out unneeded wavelengths for customized spectral outputs.

The intensity and purity of the light source directly impact the quality of data from spectrometers. These different components all serve to generate light tailored to the specific analysis needs of the instrument.

2. The Sample Holder

Once the light is ready, we need something to hold the material being analyzed – this is the sample holder or cuvette. It houses the sample the light will pass through or reflect off of. Common sample holders include:

Liquid Cuvettes

These are small transparent containers designed to hold liquid samples. Made of materials like glass or plastic, cuvettes allow light to transmit through the sample they house. Path lengths usually range from 1mm to 10mm.

Solid Sample Holders

Solids and powder samples require different holders that either reflect or diffuse light off the surface of the material into the detector. Some holders rotate or vibrate the sample for averaging.

Fiber Optic Probes

The flexible nature of fiber optics allows sample holders to be designed for hard-to-reach locations, remote areas, or measurements in extreme conditions of temperature or pressure.


Microplates contain dozens of small sample wells arranged in grids for high throughput analysis. Automated plate readers allow rapid screening of many samples in parallel.

The holder design impacts the optics, sample volumes, and total analysis time. Like choosing the right key for a lock, the sample compartment must be matched to the analysis type.

3. The Wavelength Separator

Light comprises a spectrum of wavelengths from infrared through the visible range. Spectrometers leverage this phenomenon to analyze samples’ interaction with different wavelengths. Various optical components are used to separate broad light into constituent wavelengths. Common wavelength separators include:


Prisms utilize refraction to split light into a rainbow of colors. Prisms are simple and inexpensive but lack precision. Dispersion across wavelengths is uneven.

Diffraction Gratings

Closely spaced grooves diffract light by different amounts based on wavelength. Gratings provide higher resolution than prisms. Blaze angle optimizes efficiency at certain wavelengths.


Combining dispersive elements like prisms or gratings with adjustable slits produces light of very precise bandwidths. Used as wavelength selectors with broad spectrum sources.


Colored glass filters preferentially transmit certain wavelength regions while blocking others. Simple and low cost but offer less versatility than full separators.

The wavelength separator enables the spectrometer to probe samples one wavelength region at a time, building a full spectral picture.

4. The Detector

Once the light has interacted with the sample, we need to collect it and convert the optical signal into electronic data. This is the job of the detector. It sees the transmitted, reflected, or emitted light and translates photon flux into a measurable electrical current. Common spectrometer detectors include:


These semiconductor devices generate currents proportional to light intensity. Simple, compact, and durable, photodiodes are used from the UV to near-infrared.

Photomultiplier Tubes

PMTs amplify and multiply weak optical signals to give high-sensitivity measurements. Used for low light analysis like fluorescence and chemiluminescence.

Charged Coupled Devices

CCDs are arrays of light-sensing elements providing simultaneous measurement across a range of wavelengths. This allows the acquisition of full spectra for samples.

Focal Plane Arrays

FPAs contain infrared detectors laid out in grids, allowing imaging and spectral mapping of samples. Used in techniques like FT-IR spectroscopic imaging.

The detector translates the optical interaction of the sample into numerical spectral data the computer can process. Dynamic range, sensitivity, and noise define detector performance.

5. The Optical Path

Light follows an intricate route through the spectrometer passing from source to sample to detector. Mirrors, lenses, and fibers precisely steer the beams through the system. The optical path includes:


Lenses focus light beams to maximize intensity at the sample and collector regions. Collimating lenses make rays parallel while focusing lenses converge them.


Curved mirrors assist in directing and focusing light beams with high efficiency. They impart little dispersion, unlike lenses.


Optical fibers channel light with low loss from source to sample sites and onto detectors. Fibers allow flexible configurations.


These semi-reflective mirrors divide the light, allowing a portion to reach the sample while reflecting the rest to a reference detector. This enables baseline corrections.


Adjustable slits fine-tune the size of light beams entering and exiting monochromators to achieve the desired spectral resolution.

The optical path transports light to exactly where it needs to go to complete its spectroscopic journey from generation to detection.

6. The Readout Device

The final part of the spectrometer is the device that presents the spectral data generated by the detector. It may simply display a number representing concentration or transmittance. More advanced readouts include:

Digital Panel Meters

Simple LED or LCDs numerically show the detector measurement. Used for routine analysis requiring concentration data.

Chart Recorders

These plot the spectral measurement on a paper chart over time or wavelength change. Gives hard copy output.

Computer Interfaces

USB, PCI, or ethernet cards enable detector data to be acquired by software for processing, visualization, analysis, and storage on a PC.

Wireless Transmitters

Bluetooth, Wi-Fi, or cellular modules allow spectral data to be wirelessly sent to the cloud or mobile devices. Enables remote monitoring.

The readout completes the measurement process by converting all the generated data into accessible information for the user.

Putting It All Together

We’ve toured the key components found within spectrometers, but how do they function as an integrated instrument? Here’s a summary of the complete spectroscopic process:

The light source generates photons of specific wavelengths set by the monochromator or filters. This light interacts with samples in the holder – being transmitted, reflected, or emitted. The wavelength separator diffracts this light into component bands. The detector measures the intensity of these spectral slices.

Mirrors and fibers precisely steer the light beam through this optical path from start to finish. The detector data is processed by electronics and displayed on the readout. Computers allow further analysis like peak integration and library matching.

Each component plays an indispensable role in this chained process. The light source initiates the journey, the sample holder facilitates interaction, the wavelength separator disperses, the detector captures, and the readout displays. Together these parts comprise the powerful spectroscopy arsenal enabling chemical analysis.


From tungsten bulbs to tapered optical fibers, we have demystified the inner workings of spectrometers. While they appear complex devices, spectrometers simply harness the interplay between light and matter carried out by a coordinated symphony of components.

Mastering the fundamentals of these parts provides deeper insight into instrument operation, specifications, and applications. So next time you use a spectrometer, you can appreciate the underlying physics inside!