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Conversion Tables

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Scaling up in chromatography using Linear Flow Rate

Scale-up is often an issue with protein purification. Preliminary runs are performed on small columns and then often larger amounts of product is purified on larger columns.

Because protein purification is usually performed in volume mode and not time, when scaling up it is easier to calculate the extra flow rate required for the larger column using Linear Flow Rate, at cm/hour. This takes into account the increase in the cross-sectional area of the larger column so that it will have identical flow characteristics of the small column. Attached are 2 conversion tables for the finding of Linear Flow Rate from Volumetric Flow Rate and the other way around.

Use these two tables to help you convert Volumetric Flow Rate to Linear Flow Rate and vice versa.