The purification of mRNA presents challenges due to its chemical structure and the contaminants present. These challenges, with important techniques and tips are covered in…
This Application Note from BIA-Separations looks at the hydrolysis of β-Lactoglobulin (β-Lg) by a trypsin solution and compares it to hydrolysis with trypsin immobilized on…
This Application Note from Knauer looks at a preparative method for isolating the alkaloid piperine from black peppercorns, using the compact Azura Process HPLC. The…
CIMac PrimaS™ is a new monolith column range of high performance chromatography products for analysis and purification of mRNA. The positive charge of CIMac PrimaS…
As part of KNAUER’s “Osmolalities of the World” project we thought we would look at the osmolalities of the Parramatta River and the sea outside.…
This Application Note shows the separation of twelve common cosmetics additives using HPLC and reverse phase chromatography.The additives separated were Benzyl nicotinate, Butyl p-hydroxybenzoate, 2,6-Di-tert.-butyl-4-methylphenol,…
This technical note looks at the purification of a commercial Cannabidiol (CBD) rich oil, increasing the the purity of CBD to 94.5% from 71.6%. The…
Increasing FPLC sensitivity with fluorescence-labeled proteinsThis Technical Note from Knauer looks at the difference in sensitivity between the detection of BSA monitored with UV at…
The AZURA Cannabis Purifier from Knauer is a scalable, dedicated preparative system for purifying cannabinoids from cannabis extracts. The HPLC layout consists of a pump…
Knauer’s Eurokat high performance polymer phases were especially developed for the separation of organic acids, carbohydrates, alcohols, and even complex mixtures of these compounds. Eurokat…
Knauer high pressure pumps are designed and manufactured in Germany to produce some of the finest HPLC, FPLC and dosing pumps in the world. But…
Efficient purification of cannabinoids from cannabis It is a major challenge to extract high-purity pharmaceutical active ingredients from natural products at high yields. This also…
Vanillin is a flavouring derived from orchids of the genus Vanilla, primarily from the Mexican species, flat-leaved vanilla (V. planifolia). Pre-Columbian Mesoamerican people cultivated the…
The Knauer HPLC Troubleshooting Guide When things start to go wrong HPLC can be a very frustrating technique, particularly if you are relatively new to…
Peak recycling is a method where peaks that are eluting too close to each other in an isocratic separation are reloaded back onto the column.…
“Faster to Clinical Trials – Faster to Market” What are Exosomes?Exosomes are membrane bound extracellular vesicles (EVs) that are produced in the endosomal compartment of…
Simulated moving bed (SMB) chromatography is a separation technique used in the pharmaceutical and fine chemicals industries, and in the field of bioengineering. It enables…
This application from KNAUER shows that six common cannabinoids of high medicinal interest can easily be quantified according to the monography of German Pharmacopeia. Included…
Alternaria species are common allergens in humans, growing indoors and causing hay fever or hypersensitivity reactions that sometimes leads to asthma. In addition. they readily…
BIA’s Cornerstone Exosome Pack represents a unique approach to exosome purification, for preparation of high quality research-grade exosomes, or as the foundation for clinical quality…
Here is an interesting technical note from Knauer on the use of osmolarity in the quality control of pharmaceutical solutions.The note compares the osmolality range…
Knauer has recently launched an improved version of the 2-step FPLC purification system. The new system is simplified with the use of 2 motorized Unifier…
Verification of the mycotoxin patulin from apple juice with isocratic HPLC Application Note The deleterious and mutagenic mould fungus product patulin can frame during wrong…
The determination of the osmolality of isotonic and non-isotonic beverages using a freezing point osmometer. In the last years isotonic beverages have become more and…
Describing the Basics and Principles of HPLCHere is a very good, concise article from Knauer that covers the basics of HPLC (high pressure liquid chromatography).…
We used the Knauer freezing point osmometer K-7400s To compare the osmolality at 3 points along the Parramatta River to look at the effects of…