The differences in the ratios of the four common sugars in honey, namely fructose, glucose, sucrose and maltose can help indicate whether a “honey” sample is truly honey or a substitute. Knauer has shown in this work that the ratios of these sugars can easily be determined using HPLC and hydrophilic-interaction chromatography (HILIC).
This Application Note from Knauer demonstrates the use hydrophilic-interaction chromatography (HILIC), combined with Refractive Index (RI) detection, to determine the percentage ratio of the four common sugars in honey, a honey substitute and agave nectar. A Eurospher II NH2 HILIC column was used with an isocratic elution of 80% acetonitrile, in conjunction with an AZURA® Sugar Analytical HPLC System.
For a copy (PDF 2.6 MB) of this Application Note CLICK HERE.
Sugar Analysis HPLC System
The AZURA® Sugar Analytical HPLC System from Knauer is ideally suited for the analysis of carbohydrates, but also organic acids and alcohols.
Carbohydrates, alcohols, and organic acids are important in the manufacture of many foods, pharmaceuticals, and biofuels. This dedicated system is specially designed to separate and analyze these compounds.
For more details on the AZURE range of HPLCs go to our page HPLC SYSTEMS.
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