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HPLC Separation of Additives in Cosmetics

This Application Note shows the separation of twelve common cosmetics additives using HPLC and reverse phase chromatography.

The additives separated were Benzyl nicotinate, Butyl p-hydroxybenzoate, 2,6-Di-tert.-butyl-4-methylphenol, 4-Isopropyl-3-methylphenol, Methyl p-hydroxybenzoate, Monoammonium glycyrrhizinate, Pantothenyl ethyl ether, Pyridoxine dioctanoate, Salicylic acid, Stearyl glycyrrhetinate, Trichlorocarbanilide and Tocopheryl acetate.

These were separated on a Knauer Eurospher 100-5 C18, 250 x 4.0 mm ID column with a Methanol / Water gradient, adjusted to pH 2.5 with Phosphoric acid.

CLICK HERE for a PDF copy (50KB) of this Technical Note.

Posted in Chromatography