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Purification of Cannabidiol from Cannabidiol-Rich Oil by Preparative HPLC

This technical note looks at the purification of a commercial Cannabidiol (CBD) rich oil, increasing the the purity of CBD to 94.5% from 71.6%. The main impurity was Cannabigerol (CBG) at 3.1%.

Molecular Structure of Cannabidiol
Molecular Structure of Cannabidiol
Molecular Structure of Cannabigerol
Molecular Structure of Cannabigerol

The demand of pure cannabidiol (CBD) is increasing and therefore methods are needed that allow purification of CBD in high purity from different kind of samples i.e. cannabis extract, paste or oil. Preparative HPLC is a well-established method for purification of target substances from natural substrates in high purities and was therefore applied for this task. In this application a preparative HPLC method was developed for purification of CBD from a commercial CBD rich oil. CBD was purified with high purity and recovery thus increasing the purity from 71.6% to 94.5%.

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Other related applications:

Introducing the Cannabis Purifier; the flexible Prep HPLC System for batch purification of single cannabinoids from cannabis extract

Cannabis Producer: SMB Pilot System for continuous cannabinoid production

Posted in Chromatography