When things start to go wrong HPLC can be a very frustrating technique, particularly if you are relatively new to the field. Drifting or noisy baselines, inconsistent peek shapes, tailing, and signal fade or even loss are all common problems.
To help you Knauer has published an excellent poster detailing these problems and things you can do to try and correct them; from identification of the problem to its diagnosis and problem solving, and then prevention of the problem occurring again.
Take a look!
CLICK HERE for a pdf copy of the poster (2.0 MB)