Exosomes are extracellular vesicles (EVs) that are produced in the endosomal compartment of most eukaryotic cells. In multicellular organisms, exosomes and other EVs are present in tissues and can also be found in biological fluids including blood, urine, and cerebrospinal fluid. They are also released in vitro by cultured cells into their growth medium. Exosomes are important because they can transfer molecules from one cell to another via membrane vesicle trafficking, thereby influencing the immune system, such as dendritic cells and B cells, and may play a functional role in mediating adaptive immune responses to pathogens and tumors.
The growth medium of cultured cells contains non-vesicle contaminants, especially host cell DNA and host cell proteins, so the purification of the exosomes is critical.
BIA’s Pack was designed to accommodate the sensitive requirements of exosomes during their purification:
- Achieve low shear, high capacity, and high resolution at high flow rates
- Enhance concentration and purification of exosomes
- Reduce other non-vesicle contaminants, especially host cell DNA and host cell proteins